In Vivo Transfection
Now that modern genetic techniques have allowed scientists to identify the link between specific genes and certain diseases, there is an emphasis on determining ways to alter the functioning of a gene in vivo. Strategies to treat these diseases include delivering a different gene to the cell, replacing the defective gene, or silencing the defective gene using RNAi or antisense methods.
Delivery of the therapeutic genetic molecules (DNA, RNA, Protein) is a complex problem, requiring passage across several membranes intended to keep external DNA/RNA from the intercellular counterparts. There are viral and non-viral transfection methods for delivering nucleotides to the cell cytoplasm and nucleus. Here you can find information related to DNA transformation, also called DNA cell transfection, recombinant DNA, genetic transformation, and a variety of in vivo applications, transfection services and links to commercially available in-vivo transfection reagents.
What does “In Vivo” mean?When conducting a controlled biological experiment on living cells, there are generally two main categories almost all experiments fall under: They are named, quite colloquially, “in-vitro” and “in-vivo”.
Fundamentally, in vivo includes experiments conducted on living, breathing (in one form or another) multicellular organisms. In vitro includes experiments conducted in some manner of container, usually focusing on specific, singular types of simple organisms and/or tissues. Both forms of experimentation rely on the study of living cells. It’s important to note that the difference here isn’t life and death, since both the breathing animal and the cells in the petri-dish are both technically alive.
Commercially available in vivo transfection kits – Link
Biodistribution, PF/PD, xenograft, tox/pharm preclinical research services – Link